LymphedemaV1, Main, Exploration, bibRecord, 006600

Methods for Lymphatic Vessel Culture and Gene Transfection

Identifieur interne : 006600 ( Main/Exploration ); précédent : 006599; suivant : 006601

Methods for Lymphatic Vessel Culture and Gene Transfection

Auteurs : Anatoliy A. Gashev [États-Unis] ; Michael J. Davis [États-Unis] ; Olga Y. Gasheva [États-Unis] ; Zhanna V. Nepiushchikh [États-Unis] ; Wei Wang [États-Unis] ; Patrick Dougherty [États-Unis] ; Katherine A. Kelly [États-Unis] ; Shijie Cai [Royaume-Uni] ; Pierre-Yves Von Der Weid [Canada] ; Mariappan Muthuchamy [États-Unis] ; Cynthia J. Meininger [États-Unis] ; David C. Zawieja [États-Unis]

Source :

Microcirculation [ 1073-9688 ] ; 2009-10.

RBID : ISTEX:BC921AAC6920745A79CA8C190366EBDA830393EA

Abstract

Objective: To develop the techniques needed for the specific gene/protein targeting transfection experiments in isolated lymphatic vessels, we completed two major tasks: 1) optimize the experimental conditions to maintain the viability of isolated rat lymphatic vessels in culture for sufficiently long periods of time to permit knockdown or overexpression of selected proteins/genes and 2) develop effective transfection protocols for lymphatic muscle and endothelial cells in intact lymphatic vessels without nonspecific impairment of lymphatic contractile function due to the transfection protocol itself.

Url:

DOI: 10.1080/10739680903120778

Affiliations:

Le document en format XML

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<front><div type="abstract">Objective: To develop the techniques needed for the specific gene/protein targeting transfection experiments in isolated lymphatic vessels, we completed two major tasks: 1) optimize the experimental conditions to maintain the viability of isolated rat lymphatic vessels in culture for sufficiently long periods of time to permit knockdown or overexpression of selected proteins/genes and 2) develop effective transfection protocols for lymphatic muscle and endothelial cells in intact lymphatic vessels without nonspecific impairment of lymphatic contractile function due to the transfection protocol itself.</div>
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Serveur d'exploration sur le lymphœdème

Methods for Lymphatic Vessel Culture and Gene Transfection

Methods for Lymphatic Vessel Culture and Gene Transfection

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